Publications

Comparative proteomic analysis of donor human milk treated by high-pressure processing or Holder pasteurization on undigested proteins across dynamic simulated preterm infant digestion

  • Date de publication : 2025-01-01

Référence

M. A. Pitino, D. L. O'Connor, S. Unger, J. K. Bum, A. Doyen, Md A. Wazed, S. Kumar, Y. Pouliot, D. Stone, D. C. Dallas. 2025. Comparative proteomic analysis of donor human milk treated by high-pressure processing or Holder pasteurization on undigested proteins across dynamic simulated preterm infant digestion. Food Chemistry, 432, 140973.

Information Complémentaire

Lien vers l'article: https://www.sciencedirect.com/science/article/pii/S0308814624026232 

Mot(s) Clé(s)

Lait humain Traitement non thermique Hautes pressions hydrostatiques Pasteurisation à basse température et à longue durée Digestion in-vitro Protéines bioactives

Résumé

High-pressure processing (HPP) of donor human milk (DM) minimally impacts the concentration and bioactivity of some important bioactive proteins including lactoferrin, and bile salt-stimulated lipase (BSSL) compared to Holder pasteurization (HoP), yet the impact of HPP and subsequent digestion on the full array of proteins detectable by proteomics remains unclear. We investigated how HPP impacts undigested proteins in DM post-processing and across digestion by proteomic analysis. Each pool of milk (n = 3) remained raw, or was treated by HPP (500 MPa, 10 min) or HoP (62.5 °C, 30 min), and underwent dynamic in vitro digestion simulating the preterm infant. In the meal, major proteins were minimally changed post-processing. HPP-treated milk proteins better resisted proximal digestion (except for immunoglobulins, jejunum 180 min) and the extent of undigested proteins after gastric digestion of major proteins in HPP-treated milk was more similar to raw (e.g., BSSL, lactoferrin, macrophage-receptor-1, CD14, complement-c3/c4, xanthine dehydrogenase) than HoP.

Auteur(s)

Pitino, Michael A
O'Connor, Deborah
Unger, Sharon
Bum, Jin Kim
Doyen, Alain
Wazed, Md Abdul
Kumar, Sudarshan
Pouliot, Yves
Stone, Debbie
Dallas, David C