Publications
Use of an electrodialytic reactor for the simultaneous β-lactoglobulin enzymatic hydrolysis and fractionation of generated bioactive peptides
- Date de publication : 2013-01-01
Référence
A. Doyen, É. Husson, L. Bazinet. 2013. Use of an electrodialytic reactor for the simultaneous β-lactoglobulin enzymatic hydrolysis and fractionation of generated bioactive peptides. Food Chemistry, 136, 1193-1202.
Information Complémentaire
Lien vers l'article : https://www.sciencedirect.com/science/article/abs/pii/S0308814612014203
Mot(s) Clé(s)
Beta-lactoglobuline Électrodyalise avec membrane d'ultrafiltration Hydrolyse trypsique Peptides bioactifs Lactokinine
Résumé
The enzymatic hydrolysis of β-lactoglobulin and the fractionation of peptides were performed in one step in an electrodialysis cell with ultrafiltration membranes stacked. After 240 min of treatment, 15 anionic and 4 cationic peptides were detected in the anionic and cationic peptide recovery compartments. Amongst these 15 anionic peptides, 2 hypocholesterolemic, 3 antihypertensive and 1 antibacterial peptides were recovered and concentrated with migration rates ranging from 5.5% and 81.7%. Amongst the 4 cationic peptides, the peptide sequence ALPMHIR, identified as lactokinin and known to exert an important antihypertensive effect, was recovered with an estimated 66% migration rate. To our knowledge, it was the first attempt to perform hydrolysis under an electric field and to simultaneously separate anionic and cationic peptides produced.
