Publications
- Année de publication : 2017-05-10
Référence
S. Suwal, E. Rozoy, M. Manenda, A. Doyen, L. Bazinet. 2017. Comparative study of in situ and ex situ enzymatic hydrolysis of milk protein and separation of bioactive peptides in an electromembrane reactor. ACS Sustainable Chemistry & Engineering, 5, 5330-5340.
Information Complémentaire
Lien vers l'article : https://pubs.acs.org/doi/abs/10.1021/acssuschemeng.7b00651
Mot(s) Clé(s)
In situ, Ex situ Hydrolyse enzymatique Peptides bioactifs Électrodialyse
Résumé
Tryptic hydrolysis of whey protein isolate was performed simultaneously during (in situ) and before (ex situ) fractionation by electrodialysis with ultrafiltration membrane (EDUF) to obtain bioactive peptides. Peptide migration to anionic (A(RC)(-)) and cationic (C-RC(+)) peptide recovery compartments was strongly dependent on the digestion strategy used. Indeed, peptide migration to the A(RC)(-) was observed to be higher with in situ digestion while peptide migration to the C-RC(+) was higher in an ex situ digestion: a final peptide concentration of 103.10 +/- 2.76 mu g/mL was found in the C-RC(+) (ex situ) while it was 49.65 +/- 6.13 mu g/mL in the A(RC)(-) (in situ). HPLC-MS studies showed 23 major peaks that were generated by tryptic digestion of whey protein isolate. Seven of these peptides migrated to the A(RC)(-) while nine and eight peptides migrated to the C-RC(+) for ex situ and in situ digestions, respectively. Among them, different antihypertensive, antimicrobial, and hypocholesterolemic peptides were recovered depending on the recovery compartment such as IDALNENK and VYVEELKPTPEGDLEILLQK in A(RC)(-) and ALPMHIR, TKIPAVFK, VLVLDTDYKK, and VAGTWY in C-RC(+) regardless of the mode of enzymatic hydrolysis.
